NickelMaghrebV1, Main, Exploration, bibRecord, 000907

Monolayers of natural and recombinant photosystem II on gold electrodes: Potentials for use as biosensors for detection of herbicides

Identifieur interne : 000907 ( Main/Exploration ); précédent : 000906; suivant : 000908

Monolayers of natural and recombinant photosystem II on gold electrodes: Potentials for use as biosensors for detection of herbicides

Auteurs : J. Maly [République tchèque] ; A. Masci [Italie] ; J. Masojidek [République tchèque] ; M. Sugiura [Japon] ; R. Pilloton [Italie]

Source :

Analytical letters [ 0003-2719 ] ; 2004.

RBID : Pascal:05-0048328

Descripteurs français

Pascal (Inist)
- Couche monomoléculaire, Potentiel électrode, Biodétecteur, Immobilisation, Cyanobacteria, Chélateur, Couche autoassemblée, Glutaral, Evaluation performance, Méthode électrochimique, Diffusion, Inhibiteur, Sensibilité, Inhibition, Or II, Herbicide, Nickel, NTA, Protéine, Anticorps, Sérumalbumine, Capteur chimique.
Wicri :
- topic : Herbicide, Nickel.

English descriptors

KwdEn :
- Antibody, Biosensor, Chelating agent, Chemical sensor, Cyanobacteria, Diffusion, Electrochemical method, Electrode potential, Glutaral, Gold II, Herbicide, Immobilization, Inhibition, Inhibitor, Monolayer, NTA, Nickel, Performance evaluation, Protein, Self-assembled layer, Sensitivity, Serum albumin.

Abstract

Two methods for monolayer immobilization of photosystem II (PSII) isolated from thermophilic cyanobacteria Synechococcus elongatus and prospects for its use as a biosensor for detection of herbicides are reported: (i) immobilization based on recombinant (His)₆-tagged PSII coupled with nickel-nitrilotriacetic acid (Ni-NTA) chelator monolayer on Au electrode. (ii) immobilization based on the natural PSII coupled with the protein A-anti-Dl antibody modified Au electrode. Here, Cysteamine (CYS)-self-assembled monolayer (SAM)-(NTA)-PSII monolayers were compared with traditional bovine serum albumin (BSA)-glutaraldehyde (GA)-PSII crosslinked gel matrix and better performances of the derived electrochemical biosensors were pointed out. Better diffusion of inhibitors and mediators resulted in improved sensitivity, velocity of the response, and lower I₅₀ for herbicides.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000331
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Le document en format XML

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<term>Diffusion</term>
<term>Electrochemical method</term>
<term>Electrode potential</term>
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<front><div type="abstract" xml:lang="en">Two methods for monolayer immobilization of photosystem II (PSII) isolated from thermophilic cyanobacteria Synechococcus elongatus and prospects for its use as a biosensor for detection of herbicides are reported: (i) immobilization based on recombinant (His)<sub>6</sub>
-tagged PSII coupled with nickel-nitrilotriacetic acid (Ni-NTA) chelator monolayer on Au electrode. (ii) immobilization based on the natural PSII coupled with the protein A-anti-Dl antibody modified Au electrode. Here, Cysteamine (CYS)-self-assembled monolayer (SAM)-(NTA)-PSII monolayers were compared with traditional bovine serum albumin (BSA)-glutaraldehyde (GA)-PSII crosslinked gel matrix and better performances of the derived electrochemical biosensors were pointed out. Better diffusion of inhibitors and mediators resulted in improved sensitivity, velocity of the response, and lower I<sub>50</sub>
 for herbicides.</div>
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Monolayers of natural and recombinant photosystem II on gold electrodes: Potentials for use as biosensors for detection of herbicides

Monolayers of natural and recombinant photosystem II on gold electrodes: Potentials for use as biosensors for detection of herbicides

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri